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PPM - Cytogenetic Analysis of Newly Diagnosed Acut ...
Acute Leukemia Checklist
Acute Leukemia Checklist
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Pdf Summary
The document outlines a checklist for the cytogenetic analysis of newly diagnosed acute leukemia, specifically acute myeloid leukemia (AML), acute lymphoblastic leukemia (A-ALL), and precursor B-cell lymphoblastic leukemia (P-ALL). The checklist includes several tasks and recommendations for the laboratory work-up of specimens. <br /><br />The checklist advises reviewing patient history, consulting with a pathologist for potential FISH testing, and considering that a normal result on peripheral blood specimens with less than 10% blasts may not detect an abnormal clone. It also suggests establishing two independent cultures and conducting various analyses, such as analyzing 20 cells, analyzing cells from multiple cultures, and not disregarding poor quality cells as they may represent the clone. <br /><br />The checklist further recommends preparing karyograms from stemline and sidelines, as well as preparing additional karyograms if unrelated abnormal clones are present. It emphasizes the need to distinguish between hyperdiploidy and doubling of hypodiploidy for high chromosome count cases and suggests using tailored FISH assays and genomic array to differentiate between the two. <br /><br />Specific recommendations are also provided for AML, pediatric/young adult B-ALL, pediatric/young adult ALL, adult B-ALL, Burkitt-leukemia variant ALL, and adult AML with core binding abnormalities. These recommendations include assessing gene rearrangements, performing FISH assays for specific genetic markers, and considering comprehensive mutation profiling for specific abnormalities. <br /><br />Lastly, the document emphasizes the importance of reporting PML-RARA FISH results within 24 hours. <br /><br />Overall, the checklist provides detailed guidance for conducting cytogenetic analysis in the context of newly diagnosed acute leukemia cases.
Keywords
cytogenetic analysis
acute leukemia
AML
A-ALL
P-ALL
checklist
FISH testing
independent cultures
karyograms
genomic array
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