6. HEME I: Introduction to Technology Ondemand-HEME 1 video

Video Transcription

Video Summary

The video features Adrian Jubeck, a geneticist at Brigham and Women's Hospital in Boston, discussing fish (fluorescence in situ hybridization) and its applications in hematologic malignancies. Jubeck highlights the importance of interactive learning and encourages participants to ask questions throughout the video. He explains that fish is a process used to identify specific DNA sequences in the genome by using labeled DNA or RNA probes. Jubeck discusses different types of probes used in fish, such as centromeric probes, locus-specific probes, whole chromosome paint probes, break-apart probes, and dual-color, dual-fusion probes. He provides examples and explains how to interpret the results for each probe type. Jubeck also explains the process of bringing a fish probe into the lab, including obtaining the probe, confirming its localization and specificity, establishing a normal cutoff, and carefully evaluating the probe's characteristics. He also discusses the challenges of working with fixed material and the potential limitations of fish for detecting deletions in fixed material.

Keywords

Adrian Jubeck

fish

fluorescence in situ hybridization

hematologic malignancies

DNA sequences

probe types

interpretation of results

fixed material