2023 ACMG Annual Clinical Genetics Meeting Digital Edition: Poster Gallery-A Lab Developed FISH Test for Bladder Cancer and Pancreaticobiliary Cancer

Pdf Summary

The study conducted by Wenhua Zhou and colleagues focused on the development of a Lab Developed FISH (Fluorescence in situ hybridization) test for bladder cancer and pancreaticobiliary cancer. The researchers compared their test with the UroVysion FISH method in terms of probe background and intensity.<br /><br />The study included a total of 34 control slides, from which the target range for each probe was calculated. The results showed that all four probes from the Lab Developed FISH had lower background scores than the UroVysion probes. In terms of intensity, the 9p21 probe from the Lab Developed FISH had a higher intensity score compared to the UroVysion probes.<br /><br />In addition to the comparisons, the researchers processed 60 patient cases, including 40 urine specimens and 20 pancreaticobiliary specimens. Out of these, 31 samples were tested as FISH positive and 29 samples as FISH negative by both methods.<br /><br />The study also included the use of a ChromeCheck FISH Control Slide to assess signal distribution. The results indicated that the number of signals for each probe fell within the target range on the control slide.<br /><br />Overall, the Lab Developed FISH test showed promising results in terms of probe background and intensity when compared to the UroVysion FISH method. The study supports the use of this Lab Developed FISH test as a potential diagnostic tool for bladder cancer and pancreaticobiliary cancer. Further research and validation is necessary to fully establish its effectiveness and reliability.

Asset Subtitle

Presenting Author - Wenhua Zhou, PhD; Co-Author - Tierney Murphy, CT (ASCP); Co-Author - Leslie Rowe, M.S., CT(ASCP); Co-Author - Jeff Hadley, CT (ASCP); Co-Author - Benjamin Witt, MD;

Meta Tag

Cancer Cytogenetics

FISH

Methodology

Co-Author Tierney Murphy, CT (ASCP)

Co-Author Leslie Rowe, M.S., CT(ASCP)

Co-Author Jeff Hadley, CT (ASCP)

Co-Author Benjamin Witt, MD

Presenting Author Wenhua Zhou, PhD

Keywords

Lab Developed FISH

Fluorescence in situ hybridization

bladder cancer

pancreaticobiliary cancer

UroVysion FISH method

probe background

probe intensity

ChromeCheck FISH Control Slide

diagnostic tool

research and validation